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Addgene inc shrictor
Shrictor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rictor+1+shrna/Rictor_1+shRNA+(Plasmid+%231853)/bio_rxiv__2025__03__27__645689-213-7-8
Average 93 stars, based on 28 article reviews
shrictor - by Bioz Stars, 2026-09
93/100 stars

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shRNA:

Article Title: Host cell mTORC1 is required for HCV RNA replication
Article Snippet: .. Short hairpin RNA`s (shRNA) Raptor_1 shRNA (Addgene plasmid 1857), rictor_1 shRNA (Addgene plasmid 1853), mTOR1 shRNA (Addgene plasmid 1855) scramble shRNA (Addgene plasmid 1864) and pLJM1 Flag raptor (Addgene plasmid 26633) were all purchased from Addgene, Cambridge, USA [3, 4]. ..

Article Title: The tumor suppressor TMEM127 regulates insulin sensitivity in a tissue-specific manner
Article Snippet: The following antibodies were obtained from Cell Signaling Technologies: phospho-S6 ribosomal protein/S235/236 (#2211), total S6 ribosomal protein (5G10) (#2217), phospho-AKT/S473 (#9271), phospho-AKT/T308 (#13038), total AKT (#9272), phospho-4EBP1 (##2855), Rictor (#2114), total ACACA/ACC (#3676), FASN (#3180), mTOR antibody for western (#2983) and immunoprecipitation (#2972); alpha-tubulin was from Sigma (#T9026); TMEM127 polyclonal antibody was from Bethyl laboratories (#A303-450A). .. The following plasmids were obtained from Addgene: SV40 Large-T antigen (Addgene #14088,), pCMV-VSV-G (#8454), pSPAX2 [#12260], pMD2G [#12259] pLentiCRISPRv2 [#52961], Rictor_1 shRNA (#1853) and pTM-LKO.1 (#8453). ..

Article Title: mTORC2 regulates hedgehog pathway activity by promoting stability to Gli2 protein and its nuclear translocation
Article Snippet: .. Rictor_1 shRNA (Addgene plasmid#1853) and Rictor_2 shRNA (Addgene plasmid#1854) were a gift from David Sabatini. ..

Article Title: Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells
Article Snippet: .. Knockdown was confirmed with Western blot analysis. mTOR_2 shRNA ( 85 ) (Addgene plasmid 1856), rictor_1 shRNA ( 85 ) (Addgene plasmid 1853), raptor_2 shRNA ( 85 ) (Addgene plasmid 1858), and pLKO.1 GFP shRNA (Addgene plasmid 30323) were deposited by David Sabatini (Massachusetts Institute of Technology, Cambridge, Massachusetts, USA); CMV-dR8.2 and CMV-VSVG (Addgene plasmid 8455 and 8454) were deposited by Robert Weinberg (Massachusetts Institute of Technology). ..

Article Title: mTOR kinase is a therapeutic target for respiratory syncytial virus and coronaviruses
Article Snippet: .. Scramble shRNA (1864), Raptor_1 shRNA (1857), Raptor_2 shRNA (1858), Rictor_1 shRNA (1853), Rictor_2 shRNA (1854), mTOR_1 shRNA (1855), mTOR_2 shRNA (1856) were packaged by lentiviral plasmids pMD2.G (12259) psPAX2 (12260), gifted to Addgene by Didier Trono. .. All of the above plasmids were purchase from Addgene for research purposes.

Plasmid Preparation:

Article Title: Host cell mTORC1 is required for HCV RNA replication
Article Snippet: .. Short hairpin RNA`s (shRNA) Raptor_1 shRNA (Addgene plasmid 1857), rictor_1 shRNA (Addgene plasmid 1853), mTOR1 shRNA (Addgene plasmid 1855) scramble shRNA (Addgene plasmid 1864) and pLJM1 Flag raptor (Addgene plasmid 26633) were all purchased from Addgene, Cambridge, USA [3, 4]. ..

Article Title: mTORC2 regulates hedgehog pathway activity by promoting stability to Gli2 protein and its nuclear translocation
Article Snippet: .. Rictor_1 shRNA (Addgene plasmid#1853) and Rictor_2 shRNA (Addgene plasmid#1854) were a gift from David Sabatini. ..

Article Title: Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells
Article Snippet: .. Knockdown was confirmed with Western blot analysis. mTOR_2 shRNA ( 85 ) (Addgene plasmid 1856), rictor_1 shRNA ( 85 ) (Addgene plasmid 1853), raptor_2 shRNA ( 85 ) (Addgene plasmid 1858), and pLKO.1 GFP shRNA (Addgene plasmid 30323) were deposited by David Sabatini (Massachusetts Institute of Technology, Cambridge, Massachusetts, USA); CMV-dR8.2 and CMV-VSVG (Addgene plasmid 8455 and 8454) were deposited by Robert Weinberg (Massachusetts Institute of Technology). ..

Knockdown:

Article Title: Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells
Article Snippet: .. Knockdown was confirmed with Western blot analysis. mTOR_2 shRNA ( 85 ) (Addgene plasmid 1856), rictor_1 shRNA ( 85 ) (Addgene plasmid 1853), raptor_2 shRNA ( 85 ) (Addgene plasmid 1858), and pLKO.1 GFP shRNA (Addgene plasmid 30323) were deposited by David Sabatini (Massachusetts Institute of Technology, Cambridge, Massachusetts, USA); CMV-dR8.2 and CMV-VSVG (Addgene plasmid 8455 and 8454) were deposited by Robert Weinberg (Massachusetts Institute of Technology). ..

Western Blot:

Article Title: Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells
Article Snippet: .. Knockdown was confirmed with Western blot analysis. mTOR_2 shRNA ( 85 ) (Addgene plasmid 1856), rictor_1 shRNA ( 85 ) (Addgene plasmid 1853), raptor_2 shRNA ( 85 ) (Addgene plasmid 1858), and pLKO.1 GFP shRNA (Addgene plasmid 30323) were deposited by David Sabatini (Massachusetts Institute of Technology, Cambridge, Massachusetts, USA); CMV-dR8.2 and CMV-VSVG (Addgene plasmid 8455 and 8454) were deposited by Robert Weinberg (Massachusetts Institute of Technology). ..



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